Characteristics of Collagen from Parrotfish (<i>Chlorurus Sordidus</i>), Tiger Grouper (<i>Epinephelus Fuscoguttatus</i>) and Pink Ear Emperor (<i>Lethrinus Lentjan</i>): Effect of Acetic Acid Concentration and Extraction Time

The use of marine fishes skin waste such as parrotfish (Chlorurus sordidus), tiger grouper (Epinephelus fuscoguttatus) and pink ear emperor (Lethrinus lentjan) needs improvement to increase its economic value sustainability. Therefore, this study aims investigate the effect fish acetic acid ratio with different extraction times on yield characteristics collagen obtained from three-marine (parrotfish, emperor). samples were divided into 4 groups (A, B, C D) treated sample ratios time A. [ratio 1:10 (w/v) 24 h]; B. 36 C. 1:20 D. h]. Furthermore, characterization functional was carried out using FTIR while peptide pattern analysis SDS-polyacrylamide gel electrophoresis. results showed that longer time, higher yield. Collagens three in treatment had wave spectra consistent standard amide band patterns α1 α2 chains molecular weights 93-106 kDa dominant amino acids glycine, proline alanine. They also identified type 1 similar protein spectra.